Abstract:  Lactoferricin B (Lfcin-B) is the main antimicrobial peptide generated by pepsin digestion of bovine lactoferrin (BLf). Lfcin-B also shows a significant antibacterial activities. The E. coli was used for experiment strain,to study the effect of composite sweeteners on the antibacterial activities of Lfcin-B. The results showed ,under the condition of the concentration of E.coli was 107CFU/ml and was cultured at 37℃ and pH7.0 in the peptone beef medium,that the minimum inhibitory concentration(MIC) of the purified Lfcin-B against Escherichia coli was 20μg/ml. While these sweeteners and lactoferrin acting together,the antibacterial activities of the Lfcin-B was weakened as concentration range of aspartame and sodium cyclamate and saccharin sodium was 0～5mg/ml,respectively. And the antibacterial activities of the Lfcin-B was not influenced within the concentration range of acesulfame 0～5mg/ml.

Key words:  , lactoferricin B (Lfcin-B)； composite sweeteners； antibacterial activity；
【基金】 天津市科技攻关项目(033121011)