Study on Isolation,Purification and Characteristic of Proteins in Concentrated Apple Juice

Abstract

Abstract: Proteins in concentrated apple juice were isolated and purified by ion-exchange chromatography and SDS-PAGE. The results showed that the proteins that existed in concentrated apple juice included two fractions,one is from mature apple fruit(protein Ⅰ),and the other one is enzyme which added into apple juice(protein Ⅱ). The molecular weight of protein Ⅰ is small,about 25kDa. The molecular weight of protein Ⅱis higher than 31kDa. Amino acid composition analytical of protein Ⅱ showed that the content of proline is low,protein Ⅱnot involved in post-haze directively.

Key words: concentrated apple juice, protein, isolation, purification

Li-Quan-Hong, LI Na. Study on Isolation,Purification and Characteristic of Proteins in Concentrated Apple Juice[J]. FOOD SCIENCE, 2006, 27(11): 244-248.

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Study on Isolation,Purification and Characteristic of Proteins in Concentrated Apple Juice

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