Fusion Expression and Analysis of the Epitopes in Tartary Buckwheat Allergen

Abstract

Abstract: To map the epitopes for tartary buckwheat allergenic protein (TBa) and to reveal its reaction mechanism, the TBa and epitopes gene were cloned into the expression vector pET-32m, and expressed in E.coli BL21 (DE3) host cells. Furthermore, the expression products were purified by Ni2+-NTA agarose affinity chromatography column. Analysis of SDS-PAGE and western blot indicated that the recombinant fusion proteins can be largely expressed and the 6×His tag at N-terminus were confirmed. The results of ELISA indicated that the target protein had a specific binding activity with IgE antibody in the sera of the patient allergic to buckwheat food, which should lay a useful foundation for further study of the location of the epitope and reaction mechanism of tartary buckwheat allergen.

Key words: tartary buckwheat allergen, epitope, fusion expression, immunological activity

Cai-Gui-Hong, Li-Yu-Ying, Zhang-Zheng, WANG Zhuan-Hua. Fusion Expression and Analysis of the Epitopes in Tartary Buckwheat Allergen[J]. FOOD SCIENCE, 2006, 27(11): 31-34.

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Fusion Expression and Analysis of the Epitopes in Tartary Buckwheat Allergen

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