Effect of Lutein and Zeaxanthin on Nitric Oxide Production in Mouse Peritoneal Macrophages

Abstract

Abstract: This study aimed at the relationship between lutein and zeaxanthin and NO released by macrophagesy,the mechanism of the immunomodulating activities of carotenoids. Lymphocyte and peritoneal macrophages proliferation in the presence or absence of lutein and zeaxanthin were detected with MTT assay in vitro. The percentage of phagocytosis of neutral red (NR) was detected with colorimetric assay. TNF-α in the culture supernatants were detected with biology assay. Nitric oxide (NO) production was examined by Griess reaction. The results showed: 1mg/L LPS used as stimulant,the NO release was inhibited by 5μ mol/L lutein and 2.5μmol/L zeaxanthin respectively,while NO release was enhanced when the concentration of lutein and zeaxanthin was eaceeded 10μmol/L and 5μmol/L respectively. The immunomolating function and antitumor activity of luteinand zeaxanthin may be associated with its effect on NO by macrophage in vitro.

Key words: lutein, zeaxanthin, nitricoxide, macrophage, immunomodulating activity

SUN Zhen, Xi-Hai-Yan, Chen-Zheng-Xing, Yao-Hui-Yuan. Effect of Lutein and Zeaxanthin on Nitric Oxide Production in Mouse Peritoneal Macrophages[J]. FOOD SCIENCE, 2006, 27(11): 505-509.

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Effect of Lutein and Zeaxanthin on Nitric Oxide Production in Mouse Peritoneal Macrophages

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