Optimization of the Medium for Production of Citric Acid from Aged Long-grain Non-glutinous Rice by Solid-state Fermentation

Abstract

Abstract: The effect of pretreatment of raw materials and the components of substrate on production of citric acid from aged long-grain non-glutinous rice by Aspergillus niger in solid-state fermentation was investigated. Furthermore components of substrate were optimized by the orthogonal test. The optimized pretreatment’s method of raw materials was that a particle size of ground rice was 0.5mm, the 100g ground rice of adding 60ml water was steamed for 20min at 121℃ and 40ml water was supplemented into substrate and the pH of substrate was no adjustive. The optimum components of fermentative substrate are addition 8.5g bran husk, 0.5g CaCO3 and 0.9g NH4NO3 into 100g ground rice. Under these optimized conditions, the yield of citric acid was 188g/kg DM at 25℃ for 84h.

Key words: aged long-grain non-glutinous, Aspergillus niger, solid-state fermentation, citric acid, fermentative culture medium, optimization

ZHOU Jian-xin,WU Ding,YAO Ming-lan,SHEN Hai-jin,GE Jin-rong,CHEN Mei,GU Zhen-yang. Optimization of the Medium for Production of Citric Acid from Aged Long-grain Non-glutinous Rice by Solid-state Fermentation[J]. FOOD SCIENCE, 2006, 27(12): 283-286.

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Optimization of the Medium for Production of Citric Acid from Aged Long-grain Non-glutinous Rice by Solid-state Fermentation

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