FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (12): 428-432.

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Fermentation Conditions Study of Lactase-Producing Yeast Kluyveromyces lactis

ZHANG Ming-li,LIU Wen-qun,XIONG Hua,CHEN Wang-hua,XIAO Fang,JIAN Shuang-quan   

  1. Key Laboratory of Food Science, Ministry of Education, Nanchang University, Nanchang 330047, China
  • Online:2006-12-15 Published:2011-11-23

Abstract: The fermentation culture medium of lactase-producing yeast Kluyveromyces lactis was optimized by orthogonal design and single-factor experiments. The optimal culture medium for fermentation of lactase-producing yeast Kluyveromyces lactis was shown as follows (%, W/V): lactose 8, glucose 0.5, yeast extract 0.7, urea 0.15, KH2PO4 1.5, MgSO4 0.1, MnCl2 0.01. The conditions of fermentation were also investigated in the trail and the average productivity of lactase was 1.930 ONPG unitsp erm illiliter.

Key words: lactase, Kluyveromyces lactis, fermentation conditions