Abstract: The research used human blood serum allergic to milk and non-allergic to milk and applied ELISA method to determine the binding ability between human serum and milk hydrolyzed by enzyme. The optimum conditions of hydrolyzing milk and decreasing the danger of people’s allergy and meeting the drinkable standards for human allergic to milk and dairy products were studied. The results indicated the optimum hydrolysis conditions were W(E/S)=4%, pH1.5(pepsin)and pH8.0(trypsin and papain), T=40℃(pepsin and trypsin) and 45℃(papain), t=3h. The OD value at 492nm of the binding between the hydrolyzed milk and the blood serum of allergic people to milk decreased to the OD value of the binding between the non-hydrolyzed milk and the blood serum of non-allergic people to milk. On these conditions the allergen was eliminated.

Key words: milk allergy, ELISA, allergen, HRP-sheep anti human IgE