Abstract: Monascus aurantiaaeus(As3.4384) and its mutant strains (CSM-11) were inoculated into rice, and were carried static solid-state fermentation for 14 d at 30℃. A reversed-phase high performance liquid chromatographic method has been developed for the determination of citrinin in solid-state fermentation rice. The results showed that the chromatographic separations were carried on a Symmetry C18 column by using acetonitrile-H2O (77:23, V/V, pH3.0) as mobile phases. Fluorophotomeric detection was set at λex=331nm, λem=500nm. The calibration curve was linear in the concentration range of 0.01～12.5μg/ml for citrinin in samples, and the correlation coefficients were 0.999, the limitation of detection (LOD) was 0.001μg/ml. The contents of citrinin for fermentation rice by As3.4384 and CSM-11 were 151.40, 0.218mg/kg respectively.

Key words: Monascus aurantiaaeus, mutant strain, solid-state fermentation, citrinin, rice, HPLC