FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (12): 634-636.

Previous Articles     Next Articles

Separation and Determination of Carotenoids in Orange Juice by HPLC

 HU  Jian-Zhong, WANG  Ke-Xing, PAN  Si-Yi   

  1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2006-12-15 Published:2011-11-23

Abstract: High-performance liquid chromatography with photodiode array detection was applied for separation and characterization of carotenoids from Owari. The carotenoid pigments were extracted using hexane/acetone/ethanol and saponi-fied using 10% methanolic potassium hydroxide. Two different columns were compared to determine carotenoids, more than 18 carotenoid pigments were separated with YMC C30 column.

Key words: citrus, carotenoid, pigment, HPLC