FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (12): 71-74.

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Study on the Determination of Brain AChE Activity in Crucian Carp and Its Sensitivity to Organophosphorus Pesticides

LIU Xiao-yu,HAO Qiang,WU Mou-cheng,YANG Jian,XU Ying   

  1. 1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. Key Laboratory of Ecological Environment and Resources of Inland Fisheries, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;3.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  • Online:2006-12-15 Published:2011-11-23

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Abstract: Acetylcholinesterase (AChE) was extracted from crucian brain in different growing periods and compared with each other. The activity of AChE was determined by the colorimetric method of Ellman modified by Gorun(1978). Then the optimal conditions were obtained by orthogonal test. Meanwhile, the sensitivity of AChE to three kinds of organophosphorus pesticides was studied under the optimal conditions. The results indicated that the activity of AchE from crucian with the length of 12cm was the best. The optimal conditions by orthogonal test were in the following : the amount of enzyme was 40μl, the concentration of `substrate was 120mmol/L, pH was 7.5, temperature was 35℃ and the reaction time was 15min. The sensitivity of AChE from crucian brain to organophosphorus pesticides was: dicholves>phoxime>dimethoate.

Key words: acetylcholinesterase, orthogonal test, organophosphorus pesticide, sensitivity