Abstract: This paper introduces the process of fermented health beverage, which used mulberry leaves as the main material, through yeast, acetic acid bacteria fermentation, and then juice and other complements were added. Using of the comparison tests and orthogonal tests to dentify the best conditions and formula. The best fermentation conditions are mulberry leaves powder content of 90g/L, the concentration of CaCO3 6g/L, the fermentation temperature 28～30℃ and fermenting for 2 days; The best formula is: sugar 13.2g, juice 100ml, fermented liquid 150ml/L.

Key words: mulberry leaves, fermentation, orthogonal test