Abstract: Plantaricin L-1, an anti-listeria bacteriocin, obtained by precipitation with 80% ammonium sulphate is 1280AU/ml. The mode of action is bactericidal. Influence of temperature, pH and salts on inhibitory activity of plantaricin L-1 showed: that the inhibitory activity can last 144h at lower temperature, and the CFU/ml can be decreased at higher temperatures. pH7.0 was optimal, a significant reduction in activity was observed at acidic pH values; Salts in TSBYE broth and phosphate buffer (5mmol/L, pH7.0) tested can reduce the activity of plantaricin L-1, the difference between various salts was not significant, as well as the difference between three density of the same salt. Adsorption of plantaricin L-1 was influenced by the pH in some degree, however, salts didn’t change the adsorption of plantaricin L-1.

Key words: plantaricin, L.monocytogenes, temperature, pH, salts