Optimum Extraction Process of Polysaccharides from Grateloupia filicina by Orthogonal Test

Abstract

Abstract: This paper studied the extraction process of polysaccharide from Grateloupia filicina. Single factor test and orthogonal experiment design methods L9(33) were applied to analyze the effects of the factors such as solid-liquid ratio and temperature and time on the extraction percentage of the polysaccharides. Experimental results indicated that the temperature and solid-liquid ratio would significantly affect the extraction percentage of the soluble polysaccharides. The optimum solid-liquid ratio was: 1:60, the temperature 100℃ and the extraction time 3h. In the conditions, the extraction percentage of soluble polysaccharides in Grateloupia filicina was 22.527%. No nuclear-acid and protein has been found in the polysaccharides by ultraviolet detection and Ninhydrin reaction .

Key words: , Grateloupia filicina； soluble polysaccharide； extraction technology；

Zhu-Liang, ZHANG Qing, WANG Yi-Fei, CEN Ying-Zhou. Optimum Extraction Process of Polysaccharides from Grateloupia filicina by Orthogonal Test[J]. FOOD SCIENCE, 2006, 27(2): 151-154.

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Optimum Extraction Process of Polysaccharides from Grateloupia filicina by Orthogonal Test

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