Abstract: Through using two step enrichment followed with isolation on chromogenic Enterobacter sakazakii agarose, a rapid method detection of Enterobacter sakazakii in milk-based powder infant formulae has been established in this research. Biochemical test systems such as API reagent or VITEK method were used to identify the suspicious colonies on the plates.The whole dctection process takes 72 hours and the sensitivity is about 2CFU/g, by which E nterobacter sakazakii can be effectively selected from other Enterobacter.This rapid method has good stability and easy operation, and is fit for the demands of inspection and quarantine .

Key words:  , milk-based powdered infant formulae； Enterobacter sakazakii； chromogenic Enterobacter sakazakii agarose；