Abstract: The extraction capability was studied when cellulase was extracted by reverse micellar system of gemini cationic surfactant, propilidene-1,3-bis (dimethyl -tetradecylacyloxyethylammonium bromide (Ⅱ-14-3 ), in order to study reverse micellar system of new surfactant for enzyme extraction The effects of pH, strength of ion , category of ion, the ratio of auxiliary solvent, the ratio of solvent, the concentration of enzyme and the concentration of surfactant and auxiliary surfactant (phosphatidalcholine) were systematically investigated. It was found that the best conditions for extracting the cellulase were: [NaCl]=50mmol/L, [Ⅱ-14-3]=0.3mmol/L, pH6.4, C0=0.14 and S=1.0 with the yield of extracted E as 80%. The activity of the enzyme was 93.38% that of crude enzyme after extraction. If phosphatidalcholine(PC) was added in reverse micellar system of gemini cationic surfactant(Ⅱ-14-3 ), ([Ⅱ-14-3]/[PC]=36:1), the yield of extracted E was 90% and the activity of the enzyme was 121.41% that of crude enzyme after extraction. The results could be explained by microcosmic structure of reverse micelle.

Key words:  , gemini cationic surfactant； reverse micelle； extraction； propilidene-1,3-bis(dimethyl- tetradecylacyloxyethylammonium bromide)(Ⅱ-14-3)； phosphatidalcholine(PC)； cellulase； activity of enzyme；