Desalination of Antihypertensive Peptide Extracted from Wheat Germ Protein with Macroporous Resin Column Chromatography

Abstract

Abstract: The crude antihypertensive peptides extracted from wheat germ, were purified by chromatography on macroporousresin column. The recovery rate of antihypertensive peptides was defermined by ultraviolet absorbance spectrograde, whilechlorine content was determined by potassium chromate assay method. Total desalination rate calculated by ash content,nitrogen content assessed by KJedahl method and angiotensin converting enzyme inhibitor (ACEI) activity in vitro determinedby HPLC. Results: The desalination method was simple and feasible. The maximal disposal value of macroporous resin was 12.8mg/ml, chlorine remove rate 92.31%, the total desalination rate 90.14%, and recovery rate of antihypertensive peptides morethan 85%. In average, nitrogen content increased from 5.23% to 12.34%, and in vitro ACEI activity enhanced from 47.42% to70.40%.

Key words: wheat germ, antihypertensive peptide, macroporous resin, desalination

ZHOU Cun-Shan, MA Hai-Le, YU Xiao-Jie, HE Rong-Hai. Desalination of Antihypertensive Peptide Extracted from Wheat Germ Protein with Macroporous Resin Column Chromatography[J]. FOOD SCIENCE, 2006, 27(3): 142-146.

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Desalination of Antihypertensive Peptide Extracted from Wheat Germ Protein with Macroporous Resin Column Chromatography

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