Abstract:  Solid phase extraction(SPE)and gas chromatography mass spectrometric method were applied for the determinationof thiamphenicol residues in fish samples. The fish thiamphenicol was extracted by ethyl acetate under shaker .The ethyl acetateextracts were further purified and enriched by silica and C18 solid phase extraction cartridges. The final extracts were derivatisedand thiamphenicol was quantitatively determined by gas chromatography mass spectrometry in selected ion mode(ions selected409、411、499、501). The extraction and purification conditions were optimized. The recoveries of this method forfortified samples were between 80% ～ 95%, and the relative standard deviations were between 3.6%～8.1%.The detection limitof the method was below 0.3μg/kg.

Key words:  , thiamphenicol； gas chromatography-mass spectrometry； solid phase extraction； fish；