Abstract: SYBR Green I is widely used in real-time PCR applications as an intercalating dye. The use of melting curve analysiseliminates the necessity for agarose gel electrophoresis because the melting temperature (Tm) of the specific amplicon isanalogous to the detection of an electrophoretic band. In this paper we reported the development of a duplex real-time PCR suitablefor the detection of the bar and pat genes in genetically modifed organisms, based on the intercalating dye SYBR Green I and theanalysis of the melting curves of the amplified products. Using this method, different amplification products specific for the barand pat genes were obtained and identified by their specific Tm. We have combined amplification of these products in a numberof duplex reactions and showed the suitability of the methods for identification of GMOs with a sensitivity of 0.9% in duplexreactions.

Key words:  , GMO； detection； SYBR Green I； bar； pat；