FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (3): 30-33.

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Study on the Purification of m-calpain

 HUANG  Ming, XU  Xing-Lian, MA  Han-Jun, ZHAO  Lian, ZHOU  Guang-Hong   

  1. Key Laboratory of Agricultural and Animal Products Processing and Quality Control, Ministry of Agriculture,Nanjing Agricultural University, Nanjing 210095, China
  • Online:2006-03-15 Published:2011-09-06

Abstract:  This experiment was conducted for further investigation into m-calpain activity and its role in beef aging. Fresh yellowcattle spleen (within 30min postmortem) was obtained and treated with procedures such as ultrahigh refrigeration homogenization,ionic exchange chromatography, hydrophobic chromatography, etc. The resulted m-calpain with specific activity 39.7U/mg wasverified by way of SDS-PAGE .

Key words:  , calpain system; m-calpain; purification;