FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (4): 205-207.

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Assay of Vanillin by Reversed Phase -High Pressure Liquid Chromatography

 JING  Li-Jie, HAN  Wei, YAN  Guang-Lie   

  1. 1.Department of Environmental Science and Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China;2.Department of Chemical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China
  • Online:2006-04-15 Published:2011-09-13

Abstract: A simple, rapid, accurate method is studied for assay of vanillin by Reversed Phase-High Pressure Liquid Chromatography. The chromatographic column is C18 with acetonitrile/water(85/15, V/V) as the eluting phase, and elute in isoconcentration. The detected wavelength is 279nm. The RSD is 0.46% and the recovery 98.17%~99.50%. The method is satisfactory for assaying the finished vanillin product, and also for analyzing the semifinished product .

Key words: vanillin, High Pressure Liquid Chromatography(HPLC), determination