FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (4): 208-211.

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Determination of γ-Aminobutyric Acid in Germinated Brown Rice by HPLC

 FANG  Ke-Min, LI  Zai-Gui, YUAN  Han-Cheng, MENG  Ying   

  1. 1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2.Waters China Limited—Beijing Laboratory, Beijing 100027, China
  • Online:2006-04-15 Published:2011-09-13

Abstract: A method for analysis of γ-aminobutyric acid(GABA) in germinated brown rice by high performance liquid chromatography(HPLC) was developed. A gradient elution was used for the separation and quantification of GABA pre-column derivatization with AQC. The column was Waters AccQ·Tag for free amino acids. GABA was determined with UV detector at 248.0nm. Good linearities were observed within the ranges from 0.10 to 1.0 with equation of Y=3.0×106X-7877.6 (r=0.9999). And the recoveries were 99.0%~100.5% for GABA. The method is simple, steady, sensitive, accurate. GABAs in sixteen types of germinated brown rice were determinated by the method. The results showed that the content of GABA in germinated brown rice is significantly higher than that in normal brown rice.

Key words: &gamma, -aminobutyric acid; AccQ·, Tag-derivation; HPLC; germinated brown rice;