Abstract: Resistant starch (RS) is defined as "the sum of starch and starch degradation not absorbed in the small intestine of healthy individuals". This physiological definition of RS raises a difficulty in proper analytical quantification of RS. The present review described main methods to measure RS both in vivo and in vitro. The in vitro method needed imitation in vivo conditions and to be directly validated by in vivo methods. The simple and reliable method of McCleary et al. is the only method accepted by the AOAC(AOAC method 2002.02)for determining the amount of RS in foods.

Key words: resistant starch； digestion； analytical method； pancreatic&alpha, -amylase；