Optimization of Protoplast Formation and Regeneration from an Aspergilli niger Strain Producing Acid-Stable α-Amylase

doi:10.7506/spkx1002-6630-201401030

Abstract

Abstract:

The formation and regeneration of the protoplast from Asperigillus niger with high yield of acid-stable α-amylase was Optimized using single factor experiments, Plackett-Burman design and response surface method. The optimum conditions for protoplast formation and regeneration were as follows: A. niger aged at 72h, spore concentration at 7.5×106 CFU/mL, hydrolysis with 10 g/L snailase, 10 g/L cellulose and 10 g/L lysozyme under 37℃, pH 6.8 for 2 h, and 0.85 mol/L mannitol for stabilization of osmotic pressure. At this condition, the rate of protoplast formation and regeneration reached 86.92% and 42.67 %, rescpectively.

Key words: protoplast, acid-stable α-amylase, Aspergilli niger, response surface methodology, formation, regeneration

CLC Number:

Q93.3

WANG Fa-xiang, YU Jian, WANG Jian-hui, LI Xiang-hong, HE Ji-hua, LIU Yong-le*. Optimization of Protoplast Formation and Regeneration from an Aspergilli niger Strain Producing Acid-Stable α-Amylase[J]. FOOD SCIENCE, 2014, 35(1): 155-158.

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Optimization of Protoplast Formation and Regeneration from an Aspergilli niger Strain Producing Acid-Stable α-Amylase

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