Study on Isolation, Purification and Identification of Chondroitin Sulfate-peptide

Abstract

Abstract: The crude chondroitin sulfate-peptide was extracted by papain hydrolysis, isolated and purified by detergent such as tricloroacetic acid, alcohol precipitation, dialysis and DEAE-Sepharose Fast Flow ion exchange column chromatography. Being identified by UV spectrum, IR spectrum, agarose gel electrophoresis and HPGPC, the results showed that chondroitin sulfate- peptide was predominant over the whole cartilage polysaccharide, with relevant molecular weight as 75174Da, and balanced amino acid components.

Key words: chondroitin sulfate-peptide, isolation and purification, identification

XIONG Shuang-Li, JIN Zheng-Yu. Study on Isolation, Purification and Identification of Chondroitin Sulfate-peptide[J]. FOOD SCIENCE, 2006, 27(4): 67-70.

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Study on Isolation, Purification and Identification of Chondroitin Sulfate-peptide

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