Synthesis and Identification of Immunogen 19-nortestosterone Residues in Foods

Abstract

Abstract: 19-nortestosterone (NT) is a small molecular hapten that has immunoreactivity but hasn’timmunogenicity. In this article, the 19-nortestosterone was oximated by carboxymethoxylamine and identified by MS (mass spectrograph). In order to stimulate the production of antibody, it was coupled with a carrier, bovine serum albumin (BSA),through mixed-anhydride reaction. The conjugate rate of NT and BSA was 24, identified by the analysis of spectrophotometry. Then polyclonal antibody of NT was acquired from animal immunization of this production. The dilute rate of antiserum can reach 1:102400, through an indirect enzyme-linked immunosorbent assay (ELISA), which demonstrates the synthesis of immunogen successful .And this success lays the groundwork for future researches of NT-ELISA kit.

Key words: 9-nortestosterone (NT), mixed anhydride, polyclonal antibody, ELISA, immunogen

Yu-De-He, XU Chuan-Lai, PENG Chi-Fang, WANG Wu-Kang, Guo-Jin-Hua, Jin-Zheng-Yu. Synthesis and Identification of Immunogen 19-nortestosterone Residues in Foods[J]. FOOD SCIENCE, 2006, 27(5): 195-198.

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Synthesis and Identification of Immunogen 19-nortestosterone Residues in Foods

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