Determination of Lactose and Lactulose by Ion Chromatography with Pulsed Amperometric Detection

Abstract

Abstract: In this paper, a new method for simultaneous determination of lactose and lactulose by using ion chromatography. CarboPac PA1 analytical column(2mm×250mm) and CarboPac PA1 guard column(2mm×50mm), and 5mmol/L NaOH+1mmol/L CH3COONa, stepwise elution with pulsed amperometric detection has been described. The very low conductance background could be got with the sensitivity of lactose and lactulose greatly improved. By this method, good linear relationship, sensitivity and reproducibility were obtained(r2>0.9989). The detection limits (S/N=3) of lactose and lactulose are 0.012, 0.02mg/L respectively and the recovery of sample in sample A and B ranges from 95%～102.8%。

Key words: , pulsed amperometric detection； lactose； lactulose； ion chromatography；

ZENG Wen-Fang, SHI Qiao-Cui, CHEN Yong-Xin, ZHU Jing-Ping, Zhu-Yan. Determination of Lactose and Lactulose by Ion Chromatography with Pulsed Amperometric Detection[J]. FOOD SCIENCE, 2006, 27(5): 205-207.

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Determination of Lactose and Lactulose by Ion Chromatography with Pulsed Amperometric Detection

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