Quantitative Determination of Luteolin from Flowers of Lonicera japonica by HPLC

Abstract

Abstract: Objective: The purpose is to determine the content of luteolin from the flowers of Lonicera japonica. Methods: The HPLC method was used to determine the content of luteolin from flowers of Lonicera japonica. The analysis was carried out on Shimadzu C18 column(150mm×4.6mm, 5μm).The mobile phase was MeOH-0.2% H3PO4 (50:50), flow-rate 1.0ml/min and wave-length 254nm. The column temperature was room temperature. Results: The method is simple and of a good linear relationship.The liner range of luteolin is 0.0325～0.39μg and gives a correlation (r) of 0.9999. The recovery is 99.65% and RSD 1.24%. Conclusion: The method is easy to determine the content of luteolin from the flowers of Lonicera japonica.

Key words: , Lonicerae japonicae Thunb； luteolin； HPLC；

XU Xiu-Ying, ZHENG Yi-Min, FU Shan-Quan, YANG Yan-Hong. Quantitative Determination of Luteolin from Flowers of Lonicera japonica by HPLC[J]. FOOD SCIENCE, 2006, 27(5): 221-222.

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Quantitative Determination of Luteolin from Flowers of Lonicera japonica by HPLC

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