Effects of Water Extract from Cordyceps sinensise Mycelium on SP2/0 Cell Cycles

Abstract

Abstract: After the SP2/0 cells had been treated with water extract from Cordyceps sinensise mycelium in different concentration, the effects on the growth, apoptosis, and cell cycles of the tumour cells were studied with MTT assay, agarose gel electrophoresis and flow cytometry, respectively. Results showed that the water extract (50mg/ml) has a relatively strong inhibitor effect on the proliferation of SP2/0 cells with restrain rate 70%. A typical DNA ladder was detected by agarose gel electrophoresis of DNA extracted from SP2/0 cells. Besides, the tumour cells were found to be blocked in the G0/G1 phase. It suggested that the inhibition of the tumour cells by water extract may be related to inducing the apoptosis and blocking the tumour cells in the G0/G1 phase.

Key words: , Cordyceps sinensise mycelium； water extract； SP2/0 cell； cell cycle； apoptosi.；

LIU Yan-Wei, SU Jing-Liang, HAN Bo, TIAN Xiang-Rong, ZHAO De-Ming. Effects of Water Extract from Cordyceps sinensise Mycelium on SP2/0 Cell Cycles[J]. FOOD SCIENCE, 2006, 27(5): 239-241.

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Effects of Water Extract from Cordyceps sinensise Mycelium on SP2/0 Cell Cycles

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