FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (6): 104-108.

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Study on Stability of Antimicrobial Substance Produced by Bacillus subtilis fmbR

 BIE  Xiao-Mei, 吕Feng-Xia ,   Lu-Zhao-Xin,   Wang-Dong-Li   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2006-06-15 Published:2011-09-22

Abstract: Bacillus subtilis fmbR is found to produce antimicrobial substance, which has exhibited significant inhibitory effect on Gram-positive bacteria and Gram-negative bacteria. In the paper, the stability of the antimicrobial substance on temperature, pH and three proteinases (trypsin, chymotrypsin and pepsin) was studied. The results showed that the antimicrobial substance is very stable within the tested range from 20℃ to 120℃ under pH7.0. In addition, the antimicrobial substance show no change from pH2.0~12.0 under 20℃. With regard to the three proteinases, the results indicated that the antimicrobial substance is sensitive to trypsin and chymotrypsin, but it is very stable to pepsin.

Key words:  , Bacillus subtilis fmbR; the antimicrobial substance; temperature; pH; proteinases; stability ;