Study on Extraction and Separation Technology of Chlorogenic Acid in Dandelion

Abstract

Abstract: Conditions of extraction and further isolation of chlorogenic acid from dandelion (Taraxacum. mongolicum Hand) in orthogonal test with macroporous resin NKA-9 were studied in this paper. Chlorogenic acid was isolated from dandelion with semi-preparing liquid chromatography. The optimum extraction conditions for dandelion chlorogenic acid were established as follows: ratio of material to liquor 1:16, 80% ethanol(V/V) as solvent and reflux extraction at 60℃ for 2 hours. The extraction rate of chlorogenic acid is as high as 69%. Chlorogenic acid was purified with macroporous resin NKA-9. The optimum purified conditions were established as follows: the sample concerntration 0.26mg/ml (pH 2.0), the ratio of the sample volume to the mass of macroporous resin NKA-9 12, and 30% ethanol as eluting solvent at 2ml/min. the purity of obtained chlorogenic acid is 25.3%. Finally, chlorogenic acid of higher purification (87.6%) is obtained with semi-preparing liquid chromatography.

Key words: dandelion, chlorogenic acid, abstraction, orthogonal test, separation

SHEN Qi, ZHAO Hou-Min, ZHANG Wei-Ming, LU Chang-Mei, Wu-Guo-Rong. Study on Extraction and Separation Technology of Chlorogenic Acid in Dandelion[J]. FOOD SCIENCE, 2006, 27(7): 140-144.

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Study on Extraction and Separation Technology of Chlorogenic Acid in Dandelion

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