Properties of Alcohol Dehydrogenase (ADH) Preparation from Rhizopus oryzae

Abstract

Abstract: Alcohol dehydrogenase (ADH) of Rhizopus oryzae is a kind of intracellular enzyme, it catalyzes the transformation of pyruvate to ethanol, which resulting in a decrease of pyruvate flux to target product of L-lactic acid. In this study, the crude ADH preparation was obtained from mycelium of Rhizopus oryzae As 3.3461 and its properties were investigated. The results indicated that the optimum reaction temperature of ADH is 25℃, its activity decreased rapidly when temperature was above 30℃. The pH value of reaction solution also had a marked influence on ADH activity, the optimum pH was 7.5. Addition of 1μmol EDTA, Mg2+, Ca2+ or Zn2+ to reaction solution could reduce the activity of ADH. The Km constant of ADH based on the substrata of acetaldehyde was 6.90×10-4mol/L.

Key words: , Rhizopus oryzae； alcohol dehydrogenase； enzymatic characteristics；

ZHENG Zhi, Jiang-Shao-Tong, Luo-Shui-Zhong, Pan-Li-Jun, Li-Xing-Jiang, Li-Yi-Qun. Properties of Alcohol Dehydrogenase (ADH) Preparation from Rhizopus oryzae[J]. FOOD SCIENCE, 2006, 27(9): 115-118.

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Properties of Alcohol Dehydrogenase (ADH) Preparation from Rhizopus oryzae

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