Study on Flavonoid Assay in Lithhocarpus polgstachrch Rehd with Solid Phase Extraction and HPLC

Abstract

Abstract: A high performance liquid chromatography (HPLC) method for the flavonoid assay in Lithhocarpus polgstachrch Rehd was studied. The flavonoid was extracted from the sample with 80% of ethanol by heated reflux. Then the extract was degreased by solid phase extraction with Sep-Pak-C18 cartridge. The flavonoid was separated on a Nova-Pak-C18 chromatogram column (3.9×150mm, 5μm), with methanol and 0.05mol/L potassium dihydrogen phosphate buffer solution as mobile phase at 60:40 (V/V), and monitored with the photodiode array detector at 360nm. The recoveries of the flavonoid are 97%～103%, and relative standard deviations are 0.87%～2.2%.

Key words: HPLC, solid phase extraction, Lithhocarpus polgstachrch rehd, flavonoid

YANG Ya-Ling, Zhao-Hong-Fang, Su-Yi, Zhao-Yu-Lin, Zhou-Qiang. Study on Flavonoid Assay in Lithhocarpus polgstachrch Rehd with Solid Phase Extraction and HPLC[J]. FOOD SCIENCE, 2006, 27(9): 197-199.

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Study on Flavonoid Assay in Lithhocarpus polgstachrch Rehd with Solid Phase Extraction and HPLC

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