Comparison Study on Three Methods to Assay DES Residue with HPLC,FS and CZE

Abstract

Abstract: Diethylstilbestrol is artificially produced and widely used estrogenic hormone in stockbreeding production. In the long period of using DES, the seriousness of toxic hazard to the genital system has been discovered. In this experiment the three methods of HPLC, FS, CZE were compared in order to find a highly sensitive, quick and feasible method especially suitable for detecting residue quantity of DES in animal tissues. The results showed that the minimum detectable quantity of HPLC and FS was 6.25 ×10-2 and 8.04×10-2 mg/ml respectively. The CZE was characterized by being quick, economic, simple, accurate with, littlequantity of reagent required and small liquid waste disposed, so only slightly polluted. Hence accding to the actual national conditions, the CZE is an effective and feasible method applicable in grass roots.

Key words: , diethylstilbestrol (DES)； high performance liquid chromatography(HPLC)； fluorescence spect rophotometer (FS)； capillary zone electrophoresis(CZE)；

CHEN Juan-Hua, Wang-Wen-Bo, Xu-Zai-Pin, Mao-Yi-Zhi, Lu-Zhan-Jun. Comparison Study on Three Methods to Assay DES Residue with HPLC,FS and CZE[J]. FOOD SCIENCE, 2006, 27(9): 214-218.

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Comparison Study on Three Methods to Assay DES Residue with HPLC,FS and CZE

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