Studies on Extraction of Polysaccharides from Sargassum and Its Immunocompetence

Abstract

Abstract: Purpose: To study extraction, purification and immunocompetence of Sargassum polysaccharides (sp). Methods: The SP was extracted with pretreating the Sargassum with ultrasonication for 15 minutes, then extracted 8h at 80℃, filtrated, certrifuged, condensed, and depositing the sulphated polysaccharides with alohol, and dried. Then the protein was eliminated by sevage, and the SP was purified by DEAE-cellulosecolume, dialysed, dried and doing the immunocompetence experiment. Results: The SP can increase the weight of the spleen of mice significantly (p<0.05), when the dose of polysaccharides reached respectively 20, 40 and 60mg/kg·d. It can increase the weight of the lymphonodi axillares significantly, multiplicate macrophage, quicken the cleanup speed of the lymphond nodule, strengthen the phagocytosis of monocytle-macrophages and increase the index of phagocytcis significantly(p<0.05). When the dose of polysaccharides is 40 and 60mg/kg·d, it will increase the weight of the liver of mise significantly, when the dose of polysaccharides is 60mg/kg·d.Conclusion: The SP can improve the immunocompetence.

Key words: , polysaccharides； extraction； immunocompetence；

WANG Shi-Chang, Chen-Jing, Pan-Jian-Cun, Zhou-Zhen-Bing, Li-Xing-Fang. Studies on Extraction of Polysaccharides from Sargassum and Its Immunocompetence[J]. FOOD SCIENCE, 2006, 27(9): 257-260.

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Studies on Extraction of Polysaccharides from Sargassum and Its Immunocompetence

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