FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (9): 73-75.

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Study on Cryoprotective Agents of Lactobacillus helveticus ATCC 15019 during Lyophilization

 LIU  Dan,   Pan-Dao-Dong   

  1. Department of Food Science and Nutrition, Nanjing Normal University, Nanjing 210097, China
  • Online:2006-09-15 Published:2011-10-20

Abstract:  Lactobacillus helveticus ATCC15019 is a kind of new probiotics which has the antihypertensive function. The cryoprotective agents of Lactobacillus helveticus ATCC15019 during lyophilizing were studied by single factor, orthogonal and uniform experiments. The components and dosage of the cryoprotective agents were ascertained. The results indicated that the cryoprotective agents show obvious effect on increasing the survival rate of Lactobacillus helveticus ATCC15019. When using the combination of 10.4% trehalose and 4% sodium glutamate and 11.2% skim milk , the cryoprotective agents show the most effective effect with the survival rate reaching 86.7%.

Key words:  , Lactobacillus helveticus ATCC15019; cryoprotective agents; lyophilization;