FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (9): 76-79.

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Study on Purification and Properties of Thermo-stable Xylanase from Bioengineering Bacterium 1020

 SUN  Lei,   Zhu-Xiao-Lin,   Li-Huan,   Yao-Zhong,   Wei-Ping   

  1. College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China
  • Online:2006-09-15 Published:2011-10-20

Abstract: The thermo stable xylanase from bioengineering bacterium 1020 was purified to electrophoretic homogeneity by heat treatment and Ni-NTA chromatography. The optimal conditions are: heat treated at 70℃ for 30min, and purification factor 4.9. Only one step chromatography of Ni-NTA makes xylanase up to electrophoretic homogeneity. The optimal temperature is 110℃, and can hold whole activity for 8h at 70℃ and 40% activity at 100℃ after 5h. Among the ranges from pH6.0 to pH10.0, the enzyme shows high activity. Low concentration of isopropyl alcohol accelerate enzyme activity while Hg2+ inhibits it.Mcihaelis constant of the enzyme is 0.55mg/ml, and Vmax is 14.26μmol/min·mg for the oat spelt xylan.

Key words: thermo-stable xylanase, heat treatment, Ni-NTA, separation and purification, properties