Abstract: Objective: To determine the content of adenosine, uridine and adenine in Dimocarpus longan Lour. Methods: The HPLC method was used to determine respectively the content of adenosine, uridine and adenine in Dimocarpus longan Lour. The analysis was carried out on Nucleosil C18 column(250mm×4mm). The mobile phase was 0.01mol/L KH2PO4-CH3CN (92:8, V/V), flow-rate 1.0ml/min, wavelength 254nm and temperature 40℃. Results and conclusion: The method is simple and has a good linear relationship. The linear range of adenosine is 0.1～20.0μg/ml and gives a correlation of 0.9999.The recovery of adenosine is 94.59% and RSD 3.10%. The linear range of uridine is 0.05～10.0μg/ml and gives a correlation 1. The recovery of uridine is 97.49% and RSD 4.50%. The linear range of adenine is 0.05～10.0μg/ml and gives a correlation of 1. The recovery of adenine is 103.11% and RSD 1.90%. The method is easy to determine the content of several kinds of nucleotide in Dimocarpus longan Lour by HPLC.

Key words:  , HPLC； Dimocarpus longan Lour； adenosine； uridine； adenine；