Study on Determination of Total Flavonoids in Bee-Pollen

Abstract

Abstract: Flavonoids are widely studied in natural products because of their multifarious bioactivities to human body. The paper compared tree methods of determination and confirmed a method for quantitative determination of total flavonoids in bee- pollen by ployamide-UV spectrophotometry. The standard sample was rutin. The optimum determination wavelength was 360nm. The results showed that a good linear relationship (R2=0.9998) between OD and the concentration of analyte is obtained, within the range of 0.005～0.025mg/ml. The average recovery rate is 95.53%. The stability, precision and repetition are very good, with RSD 2.124%(n=3), 0.488%(n=3), 0.875%(n=5) respectively. The content of the total flavonoids in rape pollen is 1.54% by ployamide-UV spectrophotometry.

Key words: bee-pollen, total flavonoids, ployamide-UV, colorimetry, dual wavelengh colorimetry

SUN Li-Ping, TIAN Wen-Li, ZHU Xiao-Li, DONG Jie. Study on Determination of Total Flavonoids in Bee-Pollen[J]. FOOD SCIENCE, 2007, 28(1): 262-265.

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Study on Determination of Total Flavonoids in Bee-Pollen

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