FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (10): 391-395.

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Optimization of Culture Conditions for Glutathione by Saccharomyces cerevisiae

 ZHENG  Li-Xue, HU  Yao-Hui, QI  Bin   

  1. 1.School of Food Engineering, Jilin Agricultural University, Changchun 130118, China; 2.Department of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Online:2007-10-15 Published:2011-11-22

Abstract: The production condition for glutathione by Saccharomyces cerevisiae CS10515 was investigated. The results showed that the medium composition was that, glucose 5%, yeast extract 1.2%, (NH4)2SO4 0.6%, MgSO4 0.02%, K2HPO4 0.1%. The optimal conditions for producing glutathione occured at initial pH6.0, broth volume 50%~60% and rotational speed 300 r/min. After 24 hours fermentation, the glutathione produced by Saccharomyces cerevisiae was 90.20 mg/L.

Key words: glutathione, Saccharomyces cerevisiae, biomass, fermentation