Abstract: The content of protein, polypeptides, free amino acids and distribution of molecular polypeptide weight of in BSF bioactive peptides were analyzed in this research. And the anti-oxidant activities of black bone silky fowl (BSF) bioactive peptides were evaluated by determination of the scavenging activities against hydroxyl radicals and superoxide radicals produced by Fenton reaction and pyrogallol autoxidation respectively. The results showed the protein content was 90.53% in BSF bioactive peptide, in which polypeptide amounted for 50.46% and amino acids 36.17%. The major molecular weight of BSF bioactive peptides was less than 1900 Da and small weighted peptide was dominated. BSF Bioactive peptides had a strong scavenging effect against hydroxyl radicals, and the scavenging activity showed a positive dose-response relationship with BSF bioactive peptides concentration; Also, BSF Bioactive peptide can decrease the speed of pyrogallol autoxidation, and the reducing effect showed a positive relationship with concentration, however its anti-oxidant activity in vitro is weaker than carnosine did.

Key words: black-bone silky fowl, bioactive peptides, antioxidant activity, molecular weight distribution, amino acids