Extraction and Determination of Water-soluble Polysaccharides from Peels of Grapefruit Planted in Sand-soil

Abstract

Abstract: Water-soluble polysaccharides in grapefruit peels were extracted with hot water and deposited with ethanol after removal of its impurities by refluxing with petroleum ether. Glucose was used as reference in the preparation of calibration curve. The content of water-soluble polysaccharides determined by spectrophotometry was 7.28% with 0.82% RSD(n=3). Good linearity ranged from 15.0μg/ml to 100.0μg/ml. The linear regression equation was as follows: A=0.00755X+0.07475(r=0.9992), Percent recovery of glucose from control experiment was 99.00%±1.15% with 0.59% of RSD(n=5). This method was proved to be simple, reliable and steady with reproducibility and convenient in operation. It can be used for the determination of polysaccharides in pees and pulp of grapefruit.

Key words: grapefruit, water-soluble polysaccharides, extraction, determination

LIU Guo-Cong, ZENG Chu-Jie, LIU Li-Heng, LIANG Xiao-Xian. Extraction and Determination of Water-soluble Polysaccharides from Peels of Grapefruit Planted in Sand-soil[J]. FOOD SCIENCE, 2007, 28(11): 223-226.

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Extraction and Determination of Water-soluble Polysaccharides from Peels of Grapefruit Planted in Sand-soil

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