Abstract: MRS culture medium was used for the cultivation of Lactobacillus acidophilus in this paper. Linoleate isomerase was produced with the inducement of linoleic acid added in the culture medium. Single factor experiment results showed that, the addition of saccharide has no obvious effect on enzyme activity. The addition of organic nitrogen such as urea, beef extract, casein or yeast extract can obviously improve the enzyme activity, while effects of inorganic nitrogen are not as high as organic nitrogen. Addition of certain concentration of NaCl and lecithin is also helpful for the improvement of enzyme activity. Optimal conditions determined by orthogonal test for obtaining the highest enzyme activity are as follows: cultivation temperature 37 ℃, cultivation time 24h, LA level 10 mg/100ml culture medium, and inoculation quantity 3%(V/V).

Key words:  , Lactobacillus acidophilus； linoleate isomerase； cultivation condirion for enzyme production；