Determination of Queretion in Leaves of Vacciniumu Liginosum L. by RP-HPLC

Abstract

Abstract: Determination of Quercetin in the leaves of Vacciniumu Liginosum L. was carried out. The main conditions of RP- HPLC were that: the column Shimpack VP-ODS(4.6mm×250mm, 5μm), mobile phase methanol and 0.5% of phosphoric acid (50/ 50), detecting wavelength 360 nm. The average content of quercetin was 0.78%. The results of methodology were: precision test RSD=1.13%(n=5), reproducibility test RSD=1.73%(n=5), average recovery rate of samples Y=101.6%, RSD=1.93% (n=5), which indicated that the precision, reproduction and recovery rate were proper for built of determination method.

Key words: Vacciniumu Liginosum L., quercetin, RP-HPLC

ZHANG Yan, LIN Song-Yi, LIU Jing-Bo, WANG 二Lei, SONG Long-Feng. Determination of Queretion in Leaves of Vacciniumu Liginosum L. by RP-HPLC[J]. FOOD SCIENCE, 2007, 28(11): 404-407.

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Determination of Queretion in Leaves of Vacciniumu Liginosum L. by RP-HPLC

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