FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (12): 136-138.

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Extraction and Purification of Sargassum fusiforme Polysaccharides

 JIANG  Ding-Wen, SHEN  Xian-Rong, JIA  Fu-Xing, CHEN  Wei, LIU  Li-Na   

  1. Naval Medical Research Institute, Shanghai 200433, China
  • Online:2007-12-15 Published:2011-11-22

Abstract: Objective: To study on the methods of extraction and purification polysaccharide from Sargassum fusiforme. Methods: Comparing three methods of extraction coarse polysaccharide from Sargassum fusiforme by hot water, acid hot water and hot water after degreased by ethanol. Crude polysaccharides were preliminarily purified with Sevag deprotein, and further purified by Sephadex G200 chromatography. Results: The 24.35%, 23.87% and 17.87% of crude polysaccharides were obtained from Sargassum fusiforme by these three methods, respectively. After purified, two types of protein polysaccharides were obtained from Sargassum fusiforme. The contents of their SO42-were 8.95% and 3.92%, respectively. Conclusion: Two protein polysaccharides containing sulfate were obtained from Sargassum fusiforme.

Key words: Sargassum fusiforme polysaccharides, extraction, purification