Abstract: Several methods of extraction and isolation on CoQ10 were optimized and compared. The results showed that optimum conditions of ethanol extraction were ethanol concentration 90 %, temperature 70 ℃, extraction time 120 min and ratio of material to ethanol 1:20; In process acetone grinding the optimum ratio of material to acetone is 1:3, and hexane extraction is the best; The optimum conditions of saponification extraction included acia-dissociation and saponification treatment. The former is pH 3, ratio of material to acid 1:10, tempreture 80 ℃ and reflux time 90 min. The later is pH 11, ratio of material to alkali 1: 15, tempreture 100 ℃ and reflux time 30 min. By comparing several methods of extraction, we established the optimal extraction techniques, namely acetone grinding hexane extraction.

Key words: coenzyme Q10, extraction, orthogonal test, optimization