Study on Producing Antihypertensive Peptides from Zein

Abstract

Abstract: In this study, zein, a very abundant and cheap crop in China was hydrolyzed by thermolysin, chymotrypsin, trypsin and alkaline protease for processing antihypertensive peptides. Hippuryl-Histidyl-Leucine (HHL) was used as the simulant substrate of Angiotensin Converting Enzyme (ACE), and reverse-phase performance liquid chromatography (RHPLC) was used to measure the yield of Hippuryl (Hip). According to this process, the ACE inhibitory activity of each hydrolyzate was determined and chosen as the best protease. The results showed that alkaline protease has the optimum hydrolytic capability, while the hydrolyzate of alkaline and thermolysin shows great ACE inhibitory activity (up to 90%).

Key words: zein, angiotensin converting enzyme(ACE), antihypertensive peptides

HUANG Jia-Yin, ZHU Yu-Jie, SHEN Jin-Yu. Study on Producing Antihypertensive Peptides from Zein[J]. FOOD SCIENCE, 2007, 28(12): 290-293.

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Study on Producing Antihypertensive Peptides from Zein

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