Study on Affinity Chromatography Purification of Morinda officinalis How Polysaccharides

Abstract

Abstract: The isolation of water-soluble polysaccharides (MOP) from Morinda officinalis How was discussed in detail with affinity chromatography. The static adsorption experiments of MOP revealed that the optimum conditions for the adsorption are pH 7.0 and NaCl concentration 0.1 mol/L, and optimum conditions for desorption are: pH 6.0 and the concentration of α-D-methylglucoside 0.02 mol/L. After fitting the experiments data with Langmuir equation, several adsorption parameter forMOP were obtained as: adsorption capacity qm 9. 58 mg/g, dissociation constant Kd 3.43 mg/g, and adsorption rate K 0.144 mg/h. The dynamics absorption of MOP on Con A Sepharose 4B chromatography column were investigated by breakthrough curve. Two fractions, MOP-A1 and MOP-A2 were obtained under the optimum adsorption and elution conditions.

Key words: Morinda officinalis How, polysaccharides, affinity chromatography, adsorption

JIANG Yong, LI Lin, CHEN Ling, ZHANG Xi-Mei, LI Xiao-Xi. Study on Affinity Chromatography Purification of Morinda officinalis How Polysaccharides[J]. FOOD SCIENCE, 2007, 28(12): 95-99.

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Study on Affinity Chromatography Purification of Morinda officinalis How Polysaccharides

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