FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (2): 25-28.

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Effects of Electric Field Strengths on the Ultraviolet-Visible Spectra and Activity Index of Horseradish Peroxidase

 XU  Qiang, YANG  Ti-Qiang, AO  Dun-Ge-Ri-Le   

  1. 1.College of Science and Technology, Inner Mongolia University, Huhhot 010021, China;2.Key Laboratory of Ion Beam Bioengineering of Inner Mongolia Autonomous Region, Inner Mongolia University, Hohhot 010021, China
  • Online:2007-02-15 Published:2011-12-31

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Abstract: After the horseradish peroxidase (HRP) was treated with different strengths of electric field, the HRP and activity index were assayed by ultraviolet-visible spectra. The results have shown that the HRP ultraviolet-visible spectra have been obviously changed accordingly. Experimental results also indicated that the conformation of HRP can be changed by electric field strengths and the activity index of HRP increase with certain strength of electric field. The increase of activity index might possibly be caused by the conformation changes of HRP. The results of this might have important meaning to further explore the macroscopical electrostatic biological effects.

Key words: electric field, horseradish peroxidase, ultraviolet-visible spectrum, conformation, activity index