CAP and SM2 Residues Quantitation and Fast Detection in Foodstuff by Protein Microarray

Abstract

Abstract: This study was carried about the veterinary drug residue quantitation and fast detection in foodstuff by protein microarray. The Chloramphenicol(CAP) and Sulfamethazine(SM2) synthetic-antigen coupled with the carrier protein BSA(bovine serum albumin) were spotted on the microarray. After immobilization, the antibody for these two residues and the standard orsample mixture were added.Then the Cy5 labeled secondary antibody was added after antigen-antibody reaction. The calibration curve of these two residues was established to indicate the lowest detection limit and the area the linearity range.With the calibration curve,we can calculate the concentration of the unknown sample.

Key words: protein microarray, residue, chloramphenicol(CAP), sulfamethazine（SM2）

ZUO Peng, YE Bang-Ce. CAP and SM2 Residues Quantitation and Fast Detection in Foodstuff by Protein Microarray[J]. FOOD SCIENCE, 2007, 28(2): 254-258.

[1]	ZHU Xiaoling, LIU Jie, YU Tingting, ZHANG Li, WANG Huixia, WU Wanqin, CAO Qi, ZHANG Yazhen, YANG Zong. Rapid Screening of Pesticide Residues in Pepper Powder by High Performance Liquid Chromatography Coupled with Quadrupole-Time of Flight Mass Spectrometry [J]. FOOD SCIENCE, 2021, 42(4): 287-296.
[2]	LIAN Yujing, ZHOU Yiran, SUN Xin, CHEN Jianan, ZHENG Zhenjia, WANG Minglin. Development of a Modified QuEChERS Method Based on Amine-Functionalized Iron Oxide Nanoparticles for the Simultaneous Determination of Seven Pesticides in Fruits and Vegetables by GC-MS/MS [J]. FOOD SCIENCE, 2021, 42(4): 297-304.
[3]	FU Ruijie, ZHOU Jing, TIAN Fengyu, JIAO Bining, HE Yue. Recent Progress in Nanomaterial-Based Optical Aptasensors for the Detection of Pesticide Residues [J]. FOOD SCIENCE, 2021, 42(3): 330-338.
[4]	ZHAO Lei, PAN Fei, LIN Wenxuan, XU Meili, BAO Xi, CHEN Yanlin, WANG Chengtao, LIAN Yunhe. Main Components of Stevia Residue Extract and Their Antioxidant Activities [J]. FOOD SCIENCE, 2021, 42(2): 247-254.
[5]	LIU Fengyin, LI Ying, WANG Xiaopeng, WU Xiaodan, CHEN Fengyan, LIANG Yue, YUAN Xuewen, MU Hongtao. An Enzyme Inhibition Method for Rapid Multi-residue Determination of β-Lactamase Inhibitors in Milk [J]. FOOD SCIENCE, 2021, 42(14): 248-254.
[6]	ZHONG Haowen, YANG Guoshun, CHEN Wenting, BAI Miao, TAN Jun. Determination and Pollution Characteristics of 33 Pesticide Residues in Grapes by GC-QqQ-MS/MS with Stable Isotope Internal Standard [J]. FOOD SCIENCE, 2021, 42(14): 263-269.
[7]	SHU Xiao, CHU Nengming, ZHANG Xuemei, MENG Xia, LI Dianyan, HUANG Chenglan, XIANG Jia, YANG Junying. Rapid Determination of 62 Pesticide Residues in Chicken Eggs by Enhanced Matrix Removal of Lipids Combined with Gas Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2021, 42(14): 320-327.
[8]	BAI Xue, CHEN Yu, ZHAO Lijun, ZHANG Jing. Residual Pattern and Predicting Modeling of Florfenicol in Eggs and Body Tissues of Laying Hens [J]. FOOD SCIENCE, 2021, 42(13): 26-33.
[9]	ZOU Ying, YU Yuanshan, ZOU Bo, CHENG Lina, XIAO Gengsheng, WU Jijun, BU Zhibin, XU Yujuan. Preparation and Stability of Freeze-Dried Polyphenol Extracted from Mulberry Juice Processing Residues [J]. FOOD SCIENCE, 2021, 42(1): 73-78.
[10]	LIANG Xiumei, ZHANG Weiyi, ZHANG Wei, XIE Shibing, HE Ruyi, XU Jing, SU Laijin, SUN Bolun, ZHANG Jing. Simultaneous Determination of Residues of 38 Pesticides in Fruits by QuEChERS Combined with High Performance Liquid Chromatography-Tandem Mass Spectrometry [J]. FOOD SCIENCE, 2020, 41(8): 288-296.
[11]	TONG Litao, JU Zhiyuan, GENG Donghui, WANG Lili, ZHOU Xianrong, ZHOU Sumei. Rice Vinegar Residue Reduces Adipocyte Size and Hyperlipidemia by Inhibiting Lipids Absorption in Hamsters [J]. FOOD SCIENCE, 2020, 41(5): 128-134.
[12]	MENG Yong, WANG Jing, ZHU Xiaohua, GENG Xuebing, YANG Zong, SHEN Meifang. Screening and Confirmation of Multiple Antibiotic Residues in Aquatic Products by Triple Quadrupole-Linear Ion Trap Mass Spectrometry [J]. FOOD SCIENCE, 2020, 41(4): 313-318.
[13]	ZHANG Qinjun, BEI Ronghua, ZHANG Hong, HU Changying. Determination of Residues and Migration of 17 Acrylate Monomers in Food Composite Packaging Materials by Gas Chromatography-Mass Spectrometry [J]. FOOD SCIENCE, 2020, 41(24): 287-294.
[14]	LIU Weihua, YANG Xi, LIU Minxuan, YU Wenlong, WANG Xianghong. Development of an Electrochemical Immunosensor for Rapid Quantification of Florfenicol in Animal-Derived Foods [J]. FOOD SCIENCE, 2020, 41(20): 307-313.
[15]	TONG Jixu, ZHU Zhiqiang, QIAN Yongzhong. SO2 Residue and Potential Dietary Health Risk Assessment in Grapes under Different Storage Conditions [J]. FOOD SCIENCE, 2020, 41(1): 163-167.

CAP and SM2 Residues Quantitation and Fast Detection in Foodstuff by Protein Microarray

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments