Abstract: Agrobacterium-mediated transformation has been used to introduce the Anti-PG gene into tomatoes(Lycopersicon esculentum) processing. Ten transformed tomato plants were cultivated by kanamycin-resistant experiment and regenerated plantlets were obtained from cotyledons after preculture with shoot induced culture and root induced culture. Four positive transgenic tomato plants are confirmed by PCR and Southern blot. Transgenic tomato fruits are full and lustrous. However, the Northern blot hybridization showed that the PG gene expression level of transgenic tomato is less than that of control .The phenotype shows the same result following molecular analysis.

Key words: polygalacturonase(PG), tomatoes, genetic transformation, fruit firmness